The Effect Of Ph On Enzymatic Reaction

Title : Factor Affecting Enzyme Activity (pH) Objectives 1. To read the following(a) operating instructions carefully. 2. To determine the significant effects of different pH levels on enzyme activities. 3. Find out the optimal pH execute for the reactions of the accustomed enzyme through a plot graph. Variables Independent variables : pH of root word in streak tube Dependent variables : Time for slice of colour of the outcome Constant variables : Total volume of solution in distributively test tube, volume of starch in apiece test tube, temperature of the solution. Materials Beakers, test tubes in a rack, dropper, sucker pen, pipettes, sucker, global indicator paper, stop watch, distilled water, fresh starch solution, tincture of iodine solution, amylase, 0,1mol/L hydrochloric astringent (pH=1), 0.1mol/L acetic sour (pH=3), 0.001mol/L sodium hydrated oxide (pH=11), 0.1 mol/L sodium hydroxide (pH=13), trumpery rod, grooved white tile. Procedures 1. The pH of both vitriolic and two basic was recorded in the table below. 2. 5 test tubes with the solution were set up as shown as in the table below. 3. The contents of each test tubes were complicated and the pH was checked. It was written down to the right of the first in the pH column if the pH is different from the pH recorded. 4.

every the tubes were find ad the time when the blue colour disappeared in each tube was noted. 5. The reciprocal of each time was mensural and the harbor was inserted in the 1/time column. This was used as the exercise of the enzyme. 6. A graph of activity (1/time) against pH w! as plotted on a graph paper. Results Test tubes |mL starch |mL iodine |mL amylase |mL distilled water |mL of acid or basic |pH |Time for slice of colours, t (s) |1/time (s-1) | | | | | | | |Before |After | | | |1 |3 |3 |3 |1 |0 |7 |6 |cl |0.0067 | |2 |3 |3 |3 |0 |1 mL of 0.1 mol/L HCL |1 |3 |240 |0.0042 | |3 |3 |3 |3 |0 |1 mL of 0.1 mol/L acetic acid |3 |5 |195 |0.0051 | |4 |3 |3 |3 |0 |1 mL of 0.001...If you want to get a full essay, put up it on our website: OrderCustomPaper.com

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